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Potenza Therapeutics human serum samples from healthy donors
Human Serum Samples From Healthy Donors, supplied by Potenza Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+serum+samples+from+healthy+donors/human+serum+samples+from+healthy+donors/pm30007206-46-26-16
Average 90 stars, based on 1 article reviews
human serum samples from healthy donors - by Bioz Stars, 2026-10
90/100 stars

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Article Title: A novel approach for the selective analysis of l-lysine in untreated human serum by a co-crosslinked l-lysine-α-oxidase/overoxidized polypyrrole bilayer based amperometric biosensor.
Article Snippet: Article history: Received 7 March 2018 Received in revised form 28 June 2018 Accepted 3 July 2018 Available online 06 July 2018 An amperometric biosensor based on an L-lysine-α-oxidase (LO) layer immobilized by co-crosslinking onto the surface of an overoxidized polypyrrole modified Pt electrode (Pt/oPPy) and able to analyse L-lysine (Lys) in untreated human serum is described.. The sensing electrode has been characterised and a proper enzyme kinetics optimisation permits to use a low specific enzyme as LO from Trichoderma viride for the selective biorecognition of Lys in the presence of other interferent amino acids; a kinetics study of LO evidenced also the allosteric behaviour of this enzyme, a kinetic feature which was never reported before for this enzyme.. The biosensor showed a sensitivity of 0.11 μA/mMmm, linear responses up to 4 mM and a limit of detection of 2 μM; the within-a-day coefficients of variation for replicate (n=5) were 0.92% and 1.35% at 4 mM and 0.2 mM Lys levels, respectively.



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